What is a .CXA file?
The file extension .CXA refers to a type of file known as an FMAT Listmode File. This type of file is specifically created by a software program called the FMAT 8100 HTS System. Although this program is no longer available as it has been discontinued, it was originally designed for the purpose of analyzing human cells.
A .CXA file contains detailed data collected from scanned wells containing beads. This data includes various pieces of information such as numeric identifications of each event, the location of these events within the well, the intensity of fluorescence observed, and the smoothness of these fluorescent events. Essentially, this file helps in recording and analyzing the characteristics of fluorescence in different samples, which is crucial in biological and medical research.
To open a .CXA file, you would typically need to use the Applied Biosystems FMAT 8100 HTS System software. However, since this software is discontinued, accessing these files might require specific legacy support or alternative methods. If you have the software installed, you can view the data in a .CXA file by navigating through the software menus: select File, then View Well Listmode in the Helper tool. If you have configured the FMAT software to link with Microsoft Excel, the data from the .CXA file will open in an Excel spreadsheet for easier viewing and analysis.
The files are usually stored in a specific directory structure under the "Listmode" folder, which is located within a subfolder named after the specific plate being analyzed. The naming of each .CXA file is systematic, reflecting the location of the scanned well on the plate. For example, a file named Ana05x0E.cxa would indicate that the data it contains was derived from a well located at column 5, row E of the plate.
In summary, .CXA files are specialized data files used in cell analysis applications, particularly involving fluorescence measurements. Opening and analyzing these files requires specific software, which may be challenging to access due to the discontinuation of the FMAT 8100 HTS System.
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